Select Committee on European Communities Second Report - Written Evidence


APPENDIX 3

Generic PCR test for GM food products

Genetic elementsNumber of individual
PCR tests
Identifiable
products
(total 28)

P-35S122
nos 3'1116
P-35S, nos 3'226
P-35S, nos 3', E9 3', als4 28
npt II117
P-35S, npt II2 25
P-35S, npt II, nos 3'3 26
P-nos17
P-35S, P-nos225

1The table demonstrates that by using a limited number of PCR reactions the majority of commercially available genetically modified crops can be detected.
For example, by using a combination of a P-35S primer set and a nos 3' terminator primer set, 26 out of 28 commercially available products can be identified.
P-35S = promoter from cauliflower mosaic virus (CaMV 35S);nos 3' = terminator of nopaline synthase gene (from Agrobacterium tumefaciens);E9 3' = 3' sequence of small subunit of rbcS (ribulose-1,5-bisphosphate carboxylate);als = acetolactate synthase (sulfonylurea tolerance);nptII = neomycin-3'-phosphotransferase gene from Tn-5;P-nos = promoter of nopaline synthase gene (from Agrobacterium tumefaciens).





 
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